Pilot randomised experimental study evaluating isopropyl alcohol and UVC radiation in the disinfection of healthcare workers' smartphones.

Smartphones in medical settings pose infection risks due to harbouring pathogenic bacteria. This pilot study assesses the effectiveness duration of sanitisation methods, focusing on 70% isopropyl alcohol wipes and UVC boxes, aiming to obtain preliminary data on the reduction in Total Bacterial Load 3 hours post-sanitisation. A randomised monocentric trial with two intervention arms (wipes and UVC boxes) was designed. As participants, healthcare workers from three wards at Fondazione Policlinico Universitario "A. Gemelli" IRCCS Hospital were recruited, stratified by ward, and block randomised within each ward to control confounders. Seventy-one healthcare workers, mostly nurses (62%) were included in the study. Initial bacterial load reduction was significant with both disinfection techniques, but after 3 hours both methods showed increased bacterial levels, with wipes displaying potentially higher residual efficacy (p=0.056). To adequately size a trial (89% power, significance level 0.05) for assessing the residual efficacy of alcohol-impregnated wipes compared to UVC boxes at 3 hours post-sanitisation, 503 professionals per group were required. This study highlights the necessity for guidelines on hospital smartphone sanitisation and educational initiatives for healthcare workers and patients. Further studies, adequately sized, are necessary to determine optimal sanitisation intervals and assess pathogen transmission risks.

Persistent mullerian duct syndrome in a dog.

INTRODUCTION: This case report describes a 14-year-old, male castrated York Shire Terrier, which was presented due to stranguria and tenesmus. An abdominal computed tomography (CT) scan raised a high suspicion of a persistent mullerian duct. During laparotomy structures were found that were suspected to be a persistent ovary and uterus. The abnormal uterus was filled with fluid and had a blind end on both sides. The abnormal structures were surgically removed and pathologically examined. Pathology confirmed a persistent mullerian duct. The patient recovered well from the surgery and was able to urinate spontaneously the same day and was discharged the next day.

INTRODUCTION: Ce rapport de cas décrit un York Shire Terrier mâle castré de 14 ans, qui a été présenté en raison d'une strangurie et d'un ténesme. Une tomodensitométrie (TDM) abdominale a soulevé une forte suspicion d'un canal de Müller persistant. Une laparotomie a été pratiquée, au cours de laquelle on a découvert des structures suspectées d'être un ovaire et un utérus persistants. L'utérus anormal était rempli de liquide et avait une extrémité aveugle des deux côtés. Les structures anormales ont été retirées chirurgicalement et ont fait l'objet d'un examen pathologique. La pathologie a confirmé la présence d'un canal de Müller persistant. Le patient s'est bien remis de l'opération et a pu uriner spontanément le jour même. Il a été autorisée à sortir le lendemain.

Development of a checklist as self-assessment tool to evaluate the reprocessing of endoscopic instruments in an Italian teaching hospital.

OBJECTIVE: The present study aims to develop a checklist, as a self-assessment tool, for evaluating all the items involved in the endoscope reprocessing that could be useful for the improvement and/or development of a safety endoscope reprocessing system. MATERIALS AND METHODS: A three-step modified Delphi method, with an embedded qualitative component, was adopted to develop the checklist. According to it, corrective actions were performed before its further re-administration. Contextually, the microbiological surveillance of the endoscopes and of the wash disinfector machine was carried out. RESULTS: Five areas were included in the checklist. After the 1st checklist application, only one of three wards reached the excellent scores in all the items. The other two wards showed an improvement in the Traceability and Endoscope Reprocessing areas after corrective actions. The McNemar's test reported significant difference in the proportion of satisfactory results before and after the 1st and 2nd checklist application. The microbiological surveillance, conducted after the 1st administration, showed unsatisfactory results for the 2 bronchoscopes available in the Intensive Care Unit and for 2 automated endoscope reprocessors. The analysis performed after the 2nd administration showed good results. CONCLUSIONS: The periodic administration of the checklist is functional for a self-assessment of quality reprocessing procedures carried out in the large endoscopic services and in the wards occasionally providing those services, according to the good practice guidelines and for any corrective actions to increase the safety.

Colour stability of dental restorative materials submitted to conditions of burial and drowning, for forensic purposes.

The aim of this study was to evaluate the effect of earth and water on the colour stability of tooth-coloured dental restorative materials: composite resin (CR) and glass ionomer cement (GIC). Aiming to distinguish between one and another tooth-coloured material and to estimate the period in which they could be submitted to the factors earth and water, the proposed method may contribute to the proceedings of human identification of victims of burial and submersion in water. Forty bovine incisors were prepared (6 x 6 x 2mm) and restored with CR FiltekTM Z250 XT (3M ESPETM) and GIC KetacTM Fil Plus (3M ESPETM). After initial colour read-outs (VITATM Easyshade spectrophotometer), the samples were separated into two groups (n=10), according to the conditions to which they were submitted: simulations of burying and submersion in water, for periods of 1, 3, 6 and 12 months, when new read-outs were taken. The values of colour change (ΔE, ΔL*, Δa*, and Δb*) were subjected to 3-way ANOVA statistical analysis, repeated measures, Bonferroni (p<.05), and it was verified that both factors produced colour changes in the restorative materials, which were higher for glass ionomer cement (p<.05) after 12 months of burial, and 6 months of submersion in water. The authors concluded that the analysis of colour change in the material contributed to the forensic odontology casework depending on the time during which the victim was submitted to the condition of burial or submersion in water.

Surgical treatment of a proximal diaphyseal tibial deformity associated with partial caudal and cranial cruciate ligament deficiency and patella baja.

INTRODUCTION: Caudal cruciate ligament injury can be a complication following tibial plateau leveling osteotomy (TPLO) (Slocum und Slocum, 1993) especially if the post-operative Tibial Plateau Angle (TPA) is less than 5 degree. We describe a case of negative TPA associated with partial cranial and caudal ligament rupture treated with a center of rotation of angulation (CORA) based cranial tibial opening wedge osteotomy and tibial tuberosity transposition. A 13 kg, mixed breed dog was presented for right pelvic limb lameness. Radiographically a bilateral patella baja and a malformed tibia tuberosity along with a bilateral TPA of -8 degree were detected. Arthroscopically a partial rupture of the cranial and caudal cruciate ligaments were found. A cranial tibial opening wedge osteotomy of 23 degree and a fibular ostectomy were performed. The osteotomy was fixed with a 8 holes ALPS 9 (KYON, Switzerland) and a 3-holes 2.0mm UniLock plate (Synthes, Switzerland). Then a proximal tibial tuberosity transposition of 10mm was performed and fixed with a pin and tension band construct. The postoperative TPA was 15 degree. The radiographic controls at 6, 10 weeks, 6 months and 1 year after surgery revealed an unchanged position of the implants and progressive healing of the osteotomies. At the 6 and 12 months recheck evaluation the dog had no evidence of lameness or stifle pain and radiographs revealed complete healing of the osteotomy site and no implant failure. The diaphyseal CORA based osteotomy allowed accurate correction of a proximal tibial deformity associated with negative TPA.

Influence of treatment on the outcome of dogs with incompletely excised grade-2 mast cell tumors.

INTRODUCTION: In this study we compared the outcomes of dogs with incompletely-excised grade-2 mast cell tumors (incompletely- excised grade-2 MCTs) either adjuvantly treated or not. Dogs with a grade-2 mast cell tumour (MCT) excised either incompletely or with narrow (<5mm) margins, without local recurrence or metastasis at the time of presentation and with a minimum follow-up of 10 months were included in the study. Dogs were separated in 2 groups: treatment (surgery, radiation therapy, chemotherapy or combination of those) and no-treatment. The original excision was incomplete in 90 dogs and narrow in 25 dogs. Ninety-two cases (80%) were treated and 23 (20%) were not treated, but only monitored. Pathology after revision excision found no signs of residual disease in 47/56 cases (84%). Local recurrence was confirmed in 7 dogs, suspected but not confirmed in 2 dogs. Metastatic disease was confirmed in 13 dogs and suspected but not confirmed in 11 dogs. Forty-six dogs died and 69 were still alive at the time of data collection. The 1-yr and 2-yr survival rates were 92% and 82%, respectively. No statistical differences were found regarding disease-free intervals, survival times, recurrence rates, metastatic rates, 1-year and 2-year survival rates between groups, or depending on treatment modality within the treatment group. Based on the finding that the outcome of incompletely-excised grade-2 MCTs was unaffected by adjuvant treatments, this study suggests that immediate systematic adjuvant treatment of incompletely-excised grade-2 MCTs may not be recommended over attentive monitoring and action upon uncommon recurrence.

Dans la présente étude, on compare le devenir de chiens présentant des mastocytomes de grade 2 incomplètement excisés, selon qu'ils ont reçu un traitement adjuvant ou qu'ils aient simplement été sous surveillance. On a pris en compte des chiens chez lesquels des mastocytes de grade 2 ont été incomplètement excisés ou l'ont été avec une marge étroite (<5mm) et qui ne présentaient pas de signe de récidive locale ou de métastases au moment de l'examen avec un suivi de minimum 10 mois. Les chiens ont été classés en deux groupes, l'un avec des traitements (chimiothérapie, radiothérapie, chirurgie ou combinaison de ces traitements) et l'autre sans autre traitement. L'excision originelle était incomplète chez 90 chiens et présentait des marges étroites chez 25 chiens. 92 cas (80%) ont reçu un traitement et 23 (20%) ont été surveillés sans traitement. Les résultats de l'histologie après une excision de révision n'ont pas fait état de signes d'une affection tumorale restante dans 47/56 cas (84%). Une récidive locale a été confirmée chez 7 chiens et supposée mais pas confirmée chez 2 chiens. Des métastases ont été confirmées chez 13 chiens, supposées mais non confirmées chez 11. Au moment du relevé des données, 46 chiens étaient décédés et 69 encore en vie. Le taux de survie à 1 respectivement à 2 ans était de 92% respectivement 82%. Il n'y avait pas de différence statistiquement significative entre les deux groupes en ce qui concerne les intervalles entre les affections, la durée de la survie, le taux de récidive, le taux de métastases, le taux de survie à 1 et à 2 ans ou entre les divers traitements dans le groupe des animaux traités. Les résultats de cette étude montrent que des traitements adjuvants n'influencent pas le résultat en présence de mastocytes de degré 2 incomplètement excisés. Un traitement adjuvant systématique lors de mastocytes de degré 2 incomplètement excisés ne peut donc pas être recommandé par rapport à une surveillance attentive et un traitement lors de récidive diagnostiquée.

microRNAs Modulate Spatial Memory in the Hippocampus and in the Ventral Striatum in a Region-Specific Manner.

MicroRNAs are endogenous, noncoding RNAs crucial for the post-transcriptional regulation of gene expression. Their role in spatial memory formation, however, is poorly explored. In this study, we analyzed learning-induced microRNA expression in the hippocampus and in the ventral striatum. Among miRNAs specifically downregulated by spatial training, we focused on the hippocampus-specific miR-324-5p and the ventral striatum-specific miR-24. In vivo overexpression of the two miRNAs demonstrated that miR-324-5p is able to impair memory if administered in the hippocampus but not in the ventral striatum, while the opposite is true for miR-24. Overall, these findings demonstrate a causal relationship between miRNA expression changes and spatial memory formation. Furthermore, they provide support for a regional dissociation in the post-transcriptional processes underlying spatial memory in the two brain structures analyzed.

Differential regulation of miR-21 and miR-146a by Epstein-Barr virus-encoded EBNA2.

The discovery of microRNA (miR) represents a novel paradigm in RNA-based regulation of gene expression and their dysregulation has become a hallmark of many a tumor. In virally associated cancers, the host-pathogen interaction could involve alteration in miR expression. Epstein-Barr virus (EBV)-encoded EBNA2 is indispensable for the capacity of the virus to transform B cells in vitro. Here, we studied how it affects cellular miRs. Extensive miR profiling of the virus-infected and EBNA2-transfected B lymphoma cells revealed that oncomiR miR-21 is positively regulated by this viral protein. Conversely, Burkitt's lymphoma (BL) cell lines infected with EBNA2 lacking P3HR1 strain did not show any increase in miR-21. EBNA2 increased phosphorylation of AKT and this was directly correlated with increased miR-21. In contrast, miR-146a was downregulated by EBNA2 in B lymphoma cells. Low miR-146a expression correlates with an elevated level of IRAK1 and type I interferon in EBNA2 transfectants. Taken together, the present data suggest that EBNA2 might contribute to EBV-induced B-cell transformation by altering miR expression and in particular by increasing oncomiR-like miR-21 and by affecting the antiviral responses of the innate immune system through downregulation of its key regulator miR-146a.

Epstein-Barr virus encoded LMP1 downregulates TCL1 oncogene through miR-29b.

Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is noted for its transforming potential. Yet, it also acts as a cytostatic and growth-relenting factor in Burkitt's lymphoma (BL) cells. The underlying molecular mechanisms of the growth inhibitory property of LMP1 have remained largely unknown. In this study, we show that LMP1 negatively regulates a major oncogene, TCL1, in diffuse large B-cell lymphoma (DLBCL) and BL cells. MicroRNA (miR) profiling of LMP1 transfectants showed that among others, miR-29b, is upregulated. LMP1 diminished TCL1 by inducing miR-29b through C-terminus activation region 1 (CTAR1) and CTAR2. miR-29b locked nucleic acid (LNA) antisense oligonucleotide transfection into LMP1-expressing cells reduced miR-29b expression and consequently reconstituted TCL1, suggesting that LMP1 negatively regulates TCL1 through miR-29b upregulation. The miR-29b increase by LMP1 was due to an increase in the cluster pri-miR-29b1-a transcription, derived from human chromosome 7. Using pharmacological inhibitors, we found that p38 mitogen-activated protein kinase-activating function of LMP1 is important for this effect. The ability of LMP1 to negatively regulate TCL1 through miR-29b might underlie its B-cell lymphoma growth antagonistic property. As LMP1 is also important for B-cell transformation, we suggest that the functional dichotomy of this viral protein may depend on a combination of levels of its expression, lineage and differentiation of the target cells and regulation of miRs, which then directs the outcome of the cellular response.

Substrate selectivity and pH dependence of KAAT1 expressed in Xenopus laevis oocytes.

When expressed in Xenopus oocytes KAAT1 increases tenfold the transport of l-leucine. Substitution of NaCl with 100 mm LiCl, RbCl or KCl allows a reduced but significant activation of l-leucine uptakes. Chloride-dependence is not strict since other pseudohalide anions such as thyocyanate are accepted. KAAT1 is highly sensitive to pH. It can transport l-leucine at pH 5.5 and 8, but the maximum uptake has been observed at pH 10, near to the physiological pH value, when amino and carboxylic groups are both deprotonated. The pH value mainly influences the V(max) in Na(+) activation curves and l-leucine kinetics. The kinetic parameters are K(mNa) = 4.6 +/- 2 mm, V(maxNa) = 14.8 +/- 1.7 pmol/oocyte/5 min for pH 8.0 and K(mNa) = 2. 8 +/- 0.7 mm, V(maxNa) = 31.3 +/- 1.9 pmol/oocyte/5 min for pH 10.0. The kinetic parameters of l-leucine uptake are: K(m) = 120.4 +/- 24. 2 microm, V(max) = 23.2 +/- 1.4 pmol/oocyte/5 min at pH 8.0 and K(m) = 81.3 +/- 24.2 microm, V(max) = 65.6 +/- 3.9 pmol/oocyte/5 min at pH 10.0. On the basis of inhibition experiments, the structural features required for KAAT1 substrates are: (i) a carboxylic group, (ii) an unsubstituted alpha-amino group, (iii) the side chain is unnecessary, if present it should be uncharged regardless of length and ramification.

Simultaneous measurements of ionic currents and leucine uptake at the amino acid cotransporter KAAT1 expressed in Xenopus laevis oocytes.

The transport properties of the intestinal amino acid cotransporter KAAT1, heterologously expressed in Xenopus oocytes, were studied using simultaneous voltage-clamp and tritiated leucine uptake measurements. While addition of 1 mM leucine to oocytes kept at -80 mV in presence of Na(+) or K(+) caused an increase in holding current, in presence of Li(+) the current was reduced. Uptake measurements in voltage-clamp conditions showed that a comparable accumulation of amino acid occurred in all three ionic conditions and irrespective of the direction and amount of the current change. The ratio of moles of transferred charge to moles of transported amino acid ranges from 1.45 for K(+) to 3.52 for Li(+). A hypothetical interpretation involving the coexistence of two populations of transporters, one operating in the uncoupled mode and the other in the substrate transport mode is discussed.

Ion binding and permeation through the lepidopteran amino acid transporter KAAT1 expressed in Xenopus oocytes.

1. The transient and steady-state currents induced by voltage jumps in Xenopus oocytes expressing the lepidopteran amino acid co-transporter KAAT1 have been investigated by two-electrode voltage clamp. 2. KAAT1-expressing oocytes exhibited membrane currents larger than controls even in the absence of amino acid substrate (uncoupled current). The selectivity order of this uncoupled current was Li+ > Na+ approximately Rb+ approximately K+ > Cs+; in contrast, the permeability order in non-injected oocytes was Rb+ > K+ > Cs+ > Na+ > Li+. 3. KAAT1-expressing oocytes gave rise to 'pre-steady-state currents' in the absence of amino acid. The characteristics of the charge movement differed according to the bathing ion: the curves in K+ were strongly shifted (> 100 mV) towards more negative potentials compared with those in Na+, while in tetramethylammonium (TMA+) no charge movement was detected. 4. The charge-voltage (Q-V) relationship in Na+ could be fitted by a Boltzmann equation having V of -69 +/- 1 mV and slope factor of 26 +/- 1 mV; lowering the Na+ concentrations shifted the Q-V relationship to more negative potentials; the curves could be described by a generalized Hill equation with a coefficient of 1.6, suggesting two binding sites. The maximal movable charge (Qmax) in Na+, 3 days after injection, was in the range 2.5-10 nC. 5. Addition of the transported substrate leucine increased the steady-state carrier current, the increase being larger in high K+ compared with high Na+ solution; in these conditions the charge movement disappeared. 6. Applying Eyring rate theory, the energy profile of the transporter in the absence of organic substrate included a very high external energy barrier (25.8 RT units) followed by a rather deep well (1.8 RT units).